Protein Modification Reagents
Thermo Scientific™ Pierce™ SAT(PEG)4 (PEGylated N-succinimidyl S-acetylthioacetate)
Long (18.2A), pegylated form of SATA, an amine-reactive (NHS ester) sulfhydryl-addition reagent; contains 4-unit polyethylene glycol spacer arm.
Invitrogen™ Thiol and Sulfide Quantitation Kit
Ultrasensitive colorimetric assay for quantifying both protein and nonprotein thiols
Thermo Scientific™ GlycoLink™ Coupling Catalyst
Enhance aldehyde-hydrazide crosslinking efficiency for glycoprotein immobilization and conjugation with analine (CAS 62-53-3) and an optimized buffer system.
Thermo Scientific™ MS(PEG)12 Methyl-PEG-NHS-Ester Reagent
Pegylate amines (-NH2 groups) on proteins (lysine residues) or assay surfaces using MS(PEG)n, amine-reactive (NHS ester), methyl-capped PEG compounds (4 to 24 EG units).
Thermo Scientific™ TMS(PEG)12 Branched Methyl-PEG-NHS-Ester Reagent
A three-branched, polyethylene glycol compound (3 times 12 PEG units) activated as an NHS ester for covalent PEGylation of primary amines on proteins or assay surfaces.
Thermo Scientific™ Pierce™ HRV 3C Protease Solution Kit (2 units/μL), 10,000 U PROMO
Remove fusion tags from proteins with the HRV 3C cleavage sequence with this recombinant cysteine protease that is dual tagged for easy removal from the sample after cleavage.
Thermo Scientific™ 8M Guanidine-HCl Solution
Ready-to-use, 8M solution of guanidinium hydrochloride (GdnCl, GuHCl), CAS 50-01-1; dilute and pH-adjust to any concentration for protein biology methods.
Thermo Scientific™ DTNB (Ellman's Reagent) (5,5-dithio-bis-(2-nitrobenzoic acid)
Measure peptide cysteines or other free, reduced sulfhydryls (-SH groups) in solution using this thiol-reactive, colored compound (Amax 412nm).
Thermo Scientific™ Urea
Pure, crystalline urea (CH4N2O), CAS 57-13-6; denaturant with low UV-absorptivity for protein biology methods (elution, solubilization, refolding, storage).
Gibco™ PNGase F Glycan Cleavage Kit
PNGase F Glycan Cleavage Kit includes all components necessary to perform the enzymatic removal of almost all N-linked oligosaccharides from glycoproteins.
Thermo Scientific™ Pierce™ DTT (Dithiothreitol)
Reduce disulfides in biological buffers and sample loading buffers with pure, crystalline dithiothreitol (DTT), the most popular protein disulfide reducing agent.
Thermo Scientific™ Guanidine-HCl
Pure, crystalline guanidium hydrochloride (GdnHCl, GuHCl), CAS 50-01-1; denaturant for protein biology methods (elution, solubilization, refolding, storage).
Thermo Scientific™ Trypsin, TPCK Treated
Thermo Scientific Pierce TPCK Trypsin is a serine endoprotease that is applicable to amino acid analysis and protein sequencing, mapping and structural studies; the immobilized form allows sample separation after treatment.
Thermo Scientific™ Pierce™ SATA (N-succinimidyl S-acetylthioacetate)
Short (2.8A), amine-reactive (NHS ester) sulfhydryl-addition reagent; creates stable (protected yet exposable) -SH group for protein crosslinking strategies.
Thermo Scientific Chemicals Trypsin, porcine pancreas
CAS: 7-7-9002 Molecular Formula: C35H47N7O10 Synonym: EC 3.4.21.4; Trypsin IV porcine